Determination of five compounds in Scrophularia ningpoensis by HPLC-UV-ELSD / 中国中药杂志

<p><b>OBJECTIVE</b>To develop an HPLC-UV-ELSD method for the determination of aucubi, harpagide, harpagoside, angoroside C and cinnamic acid in Scrophularia ningpoensis.</p><p><b>METHOD</b>The analytical column was SHIMADZU C18 (4.6 mm x 250 mm, 5 microm). The mobile phase was acetonirile-0.4% acetic acid in a gradient elution. Initial conditions was 5% A; 0-20 min, changed to 10% A; 20-50 min, to 55% A. The flow rate was 0.8 mL x min(-1) and the column temperature was 30 degrees C. The UV detector wavelength was set at 280 nm for harpagoside, angoroside C and cinnamic acid, and the evaporative light-scattering detector (ELSD) drift tube temperature was 105 degrees C, the flow rate of nebulizer gas was 1.2 L x min(-1) for aucubi and harpagide.</p><p><b>RESULT</b>Aucubi, harpagide, harpagoside, angoroside C and cinnamic acid was separated well. The linear calibration curves were obtained over of 0.752-13.536 microg for aucubi (r=0.9993, n=6), 0.8280-14.90 microg for harpagide (r=0.9994, n=6), 0.6360-11.45 microg for harpagoside (r=0.9997, n=6), 0.5440- 9.792 microg for angoroside C, (r=0.9997, n=6) and 0.0108-0.1939 microg for cinnamic acid (r=0.9999, n=6). The mean recovers of five compounds were 98.12%, 99.14%, 100.21%, 98.17% and 100.35% with RSD of 2.3%, 1.5%, 1.9%, 1.7% and 0.5%.</p><p><b>CONCLUSION</b>This method could simultaneously determinate the content of the five compounds in the S. ningpoensis.</p>

Effects of application of N, P and K and plant density on growth of Artemisia annua and yield of artemisinin / 中国中药杂志

<p><b>OBJECTIVE</b>To study the effects of different doses of fertilizer N,P and K and different plant densities on the growth of Artemisia annua and the yield of artemisinin which provides a scientific basis for the A. annua cultivation and artemisinin production.</p><p><b>METHOD</b>Field experiment with an orthogonal experimental design L16(45) was applied.</p><p><b>RESULT</b>N, P and K in moderate supplies increased significantly total biomass, leaf yield, artemisinin content in leaf and artemisinin yield under both per plant and per plot, with N and K having more effective on the artemisinin content in leaves than P. Ample N benefited the formation of leaf yield, but excess N had a significant negative effect on artemisinin content. Extra supply of P or K had hardly obvious father positive efficiency, nor negative on artemisinin content. The plant density had strong negative effects on total biomass, leaf output and artemisinin yield of single plant (per plant), but obvious positive effects on the same indices of population (per plot) with the moderate densities due to the enlarging numbers of the population; over crowded density significantly decreased artemisinin content and artemisinin yield per plot. By multiple comparison, the treatment 12 (N3P4K2 density 3) which produced the highest yields of artemisinin and artemisinin-rich leaf per plot was selected as the best in the experiment.</p><p><b>CONCLUSION</b>Fertilization quantities of N, P and K fertilizer and moderate density were essential for A. annua growth and artemisinin production. The optimal application of N, P and K were respectively 300 (N), 150-300(P2O5), 210(K2O) kg x hm(-2) and the optimum density was 25 000 plant x hm(-2) nearby the test area in Chongqing.</p>

Content of Medicinal Components in Artemisia annua from Different Habitats / 中国药房

OBJECTIVE:To study the contents of the medicinal components in Artemisia annua from different habitats.METHODS:A total of 22 batches of Artemisia annua samples from different habitats throughout China were collected with artemisinic acid,arteannuin B,artemisinin and scopoletin determined by HPLC.The contents of the constituents were taken as indexes to conduct correlation analysis and cluster analysis.RESULTS:The results of correlation analysis and cluster analysis showed that there were marked differences in the contents of the above-mentioned 4 constituents in Artemisia annua from various habitats.CONCLUSION:Accumulation of active ingredients of Artemisia annua is correlated to the habitats to some degree.

Optimization of taxol extraction from Taxus baccata L / 第三军医大学学报

Objective To optimize and establish the extractive technology of taxol from Taxus baccatal L.. Methods HPLC was applied to determine the content of taxol. The orthogonal test was adopted to examine the effects of the 4 factors consisting of ethanol concentration,ethanol volume,extractive times and extractive time. Results Ethanol concentration and ethanol volume were essential factors influencing the extraction of taxol. High concentration and amount of ethanol facilitated the extraction. Extractive times and extractive time were also important. By single-factorial effect,2 h and 2 times in each extraction of taxol were the best. Conclusion The optimum extractive condition is T9 (A3B3C2D1) and T8 (A3B2C1D3).

Quality standard of Xinfang Biyan Capsule / 中成药

AIM: To establish a standard for quality control of Xinfang Biyan Capsule(Radix Saponikovae, Radix Angelicae Dahuricae, Radix Scutellariae, etc.). METHODS: TLC and HPLC were used. RESULTS: Flos Magnoliae, Radix Saponikovae, Flos Chrysanthemi, Radix Bupleuri in the capsule could be identified by TLC, and baicalin content in the capsule could be measured by RP-HPLC with a linear relatioship at a range of 0.243 ?g- 2.43 ?g(r= 0.999 8 ). The average recovery was 98.15% and RSD was 0.15% . CONCLUSION: The methods are accurate and can be used for the quality control of Xinfang Biyan Capsules.